BIOQUANT OSTEO 2016 now has a standard template for the analysis of sarcopenia, the loss of muscle tissue associated with aging.
This data set is designed for use with sections that have undergone some form of muscle specific staining. Analysis of muscle tissue by H&E staining can be exceptionally tedious. Common staining methods include myosin ATPase staining, dystrophin immunohistochemistry, and dystrophin immunofluorescence. A nuclear counterstain can also be applied to identify “satellite cells” associated with the myocytes.
Stereology Estimates of Referent Data
The Stereology templates support the Stereology Tools box, which is part of the optional BIOQUANT Imaging Extensions. The protocol have been updated to support counting Bone Hits and Bone Surface Hits on the Merz grid at either low or high magnification.
The Osseointegration protocol has been updated so that contact surface can now be identified at high or low magnification using Auto Surface and Auto ROI.
Auto Surface automatically measure the length of objects. Objects can be identified by automatic thresholding or manual drawing.
Using an ROI drawn at lower magnification, Auto ROI automatically ensures that all measurements are contained within the ROI even when measuring at high magnification.
Neonatal bone sections are common tissues to study chondrocyte proliferation. Immunohistochemical PCNA labeling in paraffin sections is fairly straight-forward. BIOQUANT’s thresholding tools automatically identify PCNA+ cells based on stain color.