NEW IN BIOQUANT OSTEO 2019

Active Technical Services Plan? Request Now!

In 2019, BIOQUANT OSTEO has had two releases.

In February, a major upgrade was released, BIOQUANT OSTEO 2019 19.2.6. Users with Technical Service Plans dated February 1, 2019, and later qualify for this upgrade.

In June, a minor update was released, BIOQUANT OSTEO 2019, 19.6.6, which adds support for the Jenoptik Prokyon camera. Users with a Technical Services Plan dated June 1, 2019, and later quality for this update.

If your lab has an active Technical Services Plan, use the following buttons to read the Release Notes and Request the 2019 Upgrade. We will get you the latest version (February or June) that you qualify for.

NEW: BIOQUANT OSTEO 2019 19.6.6
June Update

This update is available to all labs with active Technical Services Plans as of June 1, 2019.

New JENOPTIK GRYPHAX® PROKYON Support

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BIOQUANT now supports the JENOPTIK GRYPHAX® PROKYON camera from Jenoptik.

Jenoptik equips the JENOPTIK GRYPHAX® PROKYON microscope cameras with the latest CMOS technology. Short exposure times and high image refresh rates make the system time-saving and convenient to use. This means you can position and focus fast and easily.

BIOQUANT is currently offering a 20% discount on the JENOPTIK GRYPHAX® PROKYON camera for the trade in of your existing camera.

FEATURES INCLUDE:

  • High image quality: 2.1, 9.2, or 20.7 megapixel image size. Pixel size 5.86 x 5.86 μm, 12 Bit, Dynamic range 73.3 dB, True color camera.

  • Sensitivity: Exposure up to 60 seconds and Gain up to 30.

  • Ease of use: Free JENOPTIK GRYPHAX® microscope camera software, large field of view 1/1.2“, 60 fps @ 2.3 MPix and no external power supply needed.

  • Stability: SONY Sensor – back illuminated, Made in Germany, 24 month warranty and software updates free of charge.

  • Live integration with BIOQUANT

  • USB 3.0 camera interface

  • In BIOQUANT, camera resolution in LIVE mode 1280 x 960 pixel (60 fps)

  • In BIOQUANT, camera resolution in HELD mode 1280 x 960 pixel, 2560x1920 pixel, or 3840 x 2880 pixel. All held image resolutions compatible with BIOQUANT live image calibration. Redraw tracings at any size. Zoom in to higher resolution at any point.

  • In GRPHAX software, camera resolution in HELD mode 1920x1200 pixel, 3840 x 2400 pixel, and 5760 x 3600 pixel.

NEW: BIOQUANT OSTEO 2019 19.2.6
February Upgrade

This upgrade is available to all labs with active Technical Services Plans as of February 28, 2019.

New TWAIN Support

TWAIN Interface

TWAIN is a standards based way of transferring images between imaging devices and imaging software. If the camera or printer/scanner in the lab has a TWAIN interface, BIOQUANT can likely connect to it now.

BIF Image Support: Display the Mag Factor

The magnification factor (the number of microns per pixel) in a BIF image is now displayed on the title bar of the Image window. This makes it easy to check the numeric value of the calibration stored in BIF images.

MAG Factor displayed on title bar of a sample image

MAG Factor displayed on title bar of a sample image

Consolidated & Improved Interface

This version of BIOQUANT has an improved user interface. Menus have been renamed for ease of use, moved to the ribbon, and/or consolidated. For a full list of interface changes, reach the PDF Release Notes.

Data Region Buttons

The Selected List region has been renamed to the Data region. The Arrays menu, the Edit menu, and parts of the File menu have been moved into the data region. Now, all the functions related to data are now found in the Data region.

Notice the Export button. It is now possible to export data to the clipboard directly from BIOQUANT!

Editing Region Buttons

The Editing region now has more tools. Buttons can be assigned to different modes. In addition, the Batch Script button has been moved to the Editing region so that all Measurement Types can run Editing scripts.

New Export Options

Data Region Export Menu

Data Region Export Menu

New Data Region allows direct export to Excel for the Data Set or the entire Project, without having to go to the Data Manager.

NEW: EXPORT DATA TABLE FOR THIS PROJECT

The “Export Data Table for this Project” option opens the Export Data Table for Volume box.

Export Data Table for this Project exports all the calculation arrays for the current data volume in a data table format to the clipboard. Then, you can click a cell in your spreadsheet program and paste.

This is ideal for projects that use the same data set template and apply it to many sections of tissue, usually using one data set per animal or patient. This is preferred because it allows you to easily audit your data and quickly track down problems.

Export Data Table for this Project gives you a simple way to create a data table. Each row contains the data for one animal and each column is a different index, either static or dynamic.

NEW: EXPORT RAW DATA FOR THIS DATA SET

The “Export Raw Data for this Data Set” option opens the Export Data Set box.

Export Raw Data for this Data Set exports all the data in the current data set, except for Topo arrays, to the clipboard. Then, you can click a cell in your spreadsheet program and paste. If you need to export Topo arrays, use the Data Manager.

NEW: EXPORT RAW DATA FOR THIS PROJECT

The “Export Raw Data for this Project” option opens the Export Data Volume box.

Export Raw Data for this Project exports all the data in the current data volume, except for Topo arrays, to the clipboard. Then, you can click a cell in your spreadsheet program and paste. If you need to export Topo arrays, use the Data Manager.

EXPORT INDIVIDUAL ARRAYS

The “Export Individual Arrays” option opens the Choose Arrays for Export box.

Previously, this was the “Available Arrays to Clipboard” box, which was opened by clicking the Edit menu and choosing Copy Data, then Choose Available Arrays.

Threshold Color Can Be Assigned to Array

The threshold color can now be assigned the array. Then, whenever the array is selected in the Selected box on the Data region, the assigned threshold color is retrieved.

New Editing Tools

The Editing region has changed quite a bit in this version. The number of tools has increased substantially. Note in particular that there are now 4 kinds of Draw tools, 4 kinds of Erase tools, 2 new Separate tools, and 2 Clean tools. Many of these new tools help with separating touching cells and are useful with the Measure Cells and Myofibers Measurement Types.

Draw and Erase Tools

Enhanced Drawing Options

Enhanced Drawing Options

New enhanced Draw tools allow faster threshold editing:

  • Freehand: Draw Free Hand (FH) enables the user to draw threshold free hand in the Image window. Click and hold the mouse button down while drawing.

  • Consecutive Distance: Draw Consecutive Distance (CD) enables the user to click consecutive points in the Image window to draw threshold. When finished with a consecutive distance series, tap the space bar to close it. Then, you can continue to click to define a new series.

  • Individual Distance: Draw Individual Distance (ID) enables the user to click two points in the Image window to draw threshold between them.

  • Star Pattern: Draw Star Pattern (SP) enables the user to click a central point in the Image window, then click points around the central point to add “arms.” Threshold is added between all the “arms” and the central point.

There is a similar Erase tool that matches the functionality of each Draw tool, but erases instead of draws threshold.

Two New Separate Tools

There are two new Separate tools that are used to separate clusters of touching cells.

Separate Tools

Separate Tools

Separate 1: Click Object to Separate

Click Object to Separate 1 is used to separate touching thresholded objects when the system can detect both separation points. When the user clicks the Separate 1 button, the system detects touching cells. It puts a yellow box and blue box at either end of separation locations. When the user clicks on accurately detected cells, they separate.

Click Object to Separate 1 is used to separate touching thresholded objects when the system can detect both separation points. When the user clicks the Separate 1 button, the system detects touching cells. It puts a yellow box and blue box at either end of separation locations. When the user clicks on accurately detected cells, they separate.

Separate 1 can be used to separate a cluster of touching cells. As you click on cells, they automatically separate and new cells to separate are detected. This allows you to separate cells that are touching other cells in a sequence. The system detects the first separate location, you click to separate, the preview outlines update and the system detects the next separate location. You repeat the sequence until all cells are separated.

Separate 1 can be used to separate a cluster of touching cells. As you click on cells, they automatically separate and new cells to separate are detected. This allows you to separate cells that are touching other cells in a sequence. The system detects the first separate location, you click to separate, the preview outlines update and the system detects the next separate location. You repeat the sequence until all cells are separated.

Separate 2: Click Second Point to Separate

Click Second Point to Separate 2 is used to separate touching thresholded objects when the system is unable to detect both separation points. When the user clicks the Separate 2 button, the system detects touching cells. It puts a yellow box on one end of the separation location. When the user clicks on second point of separation, the cell separates.

Click Second Point to Separate 2 is used to separate touching thresholded objects when the system is unable to detect both separation points. When the user clicks the Separate 2 button, the system detects touching cells. It puts a yellow box on one end of the separation location. When the user clicks on second point of separation, the cell separates.

Separate 2 is used to separate touching thresholded objects when the system is unable to detect both separation points. When the user clicks the Separate 2 button, the system detects touching cells. It puts a yellow box on one end of the separation location. When the user clicks on second point of separation, the cell separates.

Separate 2 is used to separate touching thresholded objects when the system is unable to detect both separation points. When the user clicks the Separate 2 button, the system detects touching cells. It puts a yellow box on one end of the separation location. When the user clicks on second point of separation, the cell separates.

New Keyboard Shortcuts

Hot Key: U

While using the Editing tools in the Image window, it’s now possible to tap the U key to undo the most recent change to the threshold. Previously, it was necessary to exit the Image window and click the Undo button, which would reset the threshold to its appearance when the cursor last entered the Image window.

For example, if you are editing the threshold in the Image window and have done 10 edits and make a mistake on the 11th, you can tap the “U” key and only the 11th editing command is undone. Previously, if you right clicked to exit the Image window and clicked the Undo button, all 11 edit commands would have been undone.

Hot Keys: V B N M

Editing tool hot keys have been expanded. These keys, accessible in a single row with the left hand, switch among the Separate (V), Draw (B), Erase (N), and Fill (M) buttons. These keys were chosen because of their convenient location, not necessarily because of they matched the command names.

Editing tool hot keys have been expanded. These keys, accessible in a single row with the left hand, switch among the Separate (V), Draw (B), Erase (N), and Fill (M) buttons. These keys were chosen because of their convenient location, not necessarily because of they matched the command names.

Hot Keys: 1 2 3 4

These keys, accessible in a single row with the left hand, switch among the different modes of the of the Draw and Erase tools. Freehand (1), Consecutive Distance (2), Individual Distance (3), Star Pattern (4).

These keys, accessible in a single row with the left hand, switch among the different modes of the of the Draw and Erase tools. Freehand (1), Consecutive Distance (2), Individual Distance (3), Star Pattern (4).