Neonatal sections are common tissues to study chondrocyte proliferation. Immunohistochemical PCNA labeling in paraffin sections is fairly straight-forward. BIOQUANT’s thresholding tools automatically identify PCNA+ cells based on stain color.
BIOQUANT Life Science 2016 now has a standard template for the analysis of muscle diseases, from muscular dystrophy to sarcopenia to cachexia.
This data set is designed for use with sections that have undergone some form of muscle specific staining. Analysis of muscle tissue by H&E staining can be exceptionally tedious. Common staining methods include myosin ATPase staining, dystrophin immunohistochemistry, and dystrophin immunofluorescence. A nuclear counterstain can also be applied to identify “satellite cells” associated with the myocytes.
Spinal Core Pathology
BIOQUANT LIFE SCIENCE provides standardized templates for the analysis of cell number and cellular distribution. Advanced thresholding tools allow you to mix automated thresholding with manual drawing to yield precise measurements.
By zooming to the minimum necessary resolution, a protocol with BIOQUANT can be greatly accelerated. Each field of view analyzes the the maximum amount of tissue possible for the type to data required.
Automatic regions of interest for any field of view, based on tracings of the measurement region made a low magnification, ensure data are collected only within the proper anatomic region.