BIOQUANT OSTEO has a host of new features that simplify and accelerate data collection for skeletal phenotyping.
Sarcopenia: Age Related Muscle Loss
BIOQUANT OSTEO now has a standard template for the phenotyping of skeletal muscle.
This data set is designed for use with sections that have undergone some form of muscle specific staining. Analysis of muscle tissue by H&E staining can be exceptionally tedious. Common staining methods include myosin ATPase staining, dystrophin immunohistochemistry, and dystrophin immunofluorescence. A nuclear counterstain can also be applied to identify “satellite cells” associated with the myocytes.
The Osseointegration protocol has been updated so that contact surface can now be identified at high or low magnification using Auto Surface and Auto ROI.
Auto Surface automatically measure the length of objects. Objects can be identified by automatic thresholding or manual drawing.
Using an ROI drawn at lower magnification, Auto ROI automatically ensures that all measurements are contained within the ROI even when measuring at high magnification.
Neonatal bone sections are common tissues to study chondrocyte proliferation. Immunohistochemical PCNA labeling in thin sections is fairly straight-forward. BIOQUANT’s thresholding tools automatically identify PCNA+ cells based on stain color.