Top 10 Reasons to Upgrade to BIOQUANT OSTEO 2019

Is your lab using an earlier version of BIOQUANT? Need to justify an upgrade to the latest version? Here are our 10 best reasons to upgrade to the latest version of BIOQUANT OSTEO.


Reason #1: Faster Digital Cameras


BIOQUANT now supports the JENOPTIK GRYPHAX® PROKYON camera from Jenoptik.


Jenoptik equips the JENOPTIK GRYPHAX® PROKYON microscope cameras with the latest CMOS technology. Short exposure times and high image refresh rates make the system time-saving and convenient to use. This means you can position and focus fast and easily.

BIOQUANT is currently offering a 20% discount on the JENOPTIK GRYPHAX® PROKYON camera for the trade in of your existing camera.


  • High image quality: 2.1, 9.2, or 20.7 megapixel image size. Pixel size 5.86 x 5.86 μm, 12 Bit, Dynamic range 73.3 dB, True color camera.

  • Sensitivity: Exposure up to 60 seconds and Gain up to 30.

  • Ease of use: Free JENOPTIK GRYPHAX® microscope camera software, large field of view 1/1.2“, 60 fps @ 2.3 MPix and no external power supply needed.

  • Stability: SONY Sensor – back illuminated, Made in Germany, 24 month warranty and software updates free of charge.

  • Live integration with BIOQUANT

  • USB 3.0 camera interface

  • In BIOQUANT, camera resolution in LIVE mode 1280 x 960 pixel (60 fps)

  • In BIOQUANT, camera resolution in HELD mode 1280 x 960 pixel, 2560x1920 pixel, or 3840 x 2880 pixel. All held image resolutions compatible with BIOQUANT live image calibration. Redraw tracings at any size. Zoom in to higher resolution at any point.

  • In GRPHAX software, camera resolution in HELD mode 1920x1200 pixel, 3840 x 2400 pixel, and 5760 x 3600 pixel.


Reason #2: Faster Modern Protocols


Our constant goal at BIOQUANT is to improve the speed of data collection.All of these templates have been continuously improved. Many steps have been eliminating, making data collection faster. Most recent improvements are to:

  • Skeletal Phenotyping

  • Skeletal Muscle Phenotyping

  • Human Bone Biopsy Analysis

  • Osseointegration Analysis

  • Artiritis Bone and Cartilage Analysis

  • Cancer Metastasis to Bone

  • Stereology Estimate of Referent Data

Example Template improvements


Reason #3 Faster Thresholding

Threshold: Basis of Automated Measurement
An accurate threshold is the key to automated measurement. BIOQUANT has made significant progress in automating the creation of accurate thresholds.

Scroll Wheel threshold Adjustment

Fine-tune the Threshold By Scrolling the Mouse Wheel
Starting with the initial color, scrolling the mouse wheel up will search the image for similar colors and add them to the threshold automatically. Scrolling the mouse wheel down will find the colors in the threshold that are least similar to the initial color and remove them automatically.


Automatically Remove Marrow Threshold
The "Clean" button searches the image for all "small" bits of threshold and deletes them. This makes it quick to isolate the important parts of an image from the surrounding tissue despite non-specific background staining.

Keep Just What you Want
The "Keep" button allows you to mark just the parts of the threshold you need and then deletes the rest.

Multi-Band Thresholding

True Polychromatic Detection
Our latest tool allows exact detection in multi-chromatic staining like trichrome. A single threshold can now be constructed from multiple distinct colors. For example, mineralized bone and osteoid in animal models of osteomalacia.

Previously, the threshold was limited to a single color and its light and dark shades.


Reason#4: Faster Imaging

BIOQUANT SCAN: EASY Digital pathology

BIOQUANT SCAN controls a motorized stage with the help of an autofocus camera to automatically scan histology slides or well plate cultures. Analysis of digital scans is faster to perform and easier to train.

BIOQUANT can zoom out to measure referent data at one time for the entire section. Moving around a high magnification is faster and simpler when all you need to do is drag a box around the Large Image Navigator.

4GB Support for digital scans in TIF, BMP, & BIF Format

With the Imaging Extensions, BIOQUANT support scans up to 4GB in TIF or BIF image formats. This makes it easy to work with digital slides from BIOQUANT SCAN and many other digital pathology scanners. With a 20X lens, that's more than 300mm2 of tissue. This accomodates sections up to the size of standard human biopsy.

Having access to the entire section, means BIOQUANT can zoom out to rapidly measure large structures like TV, BV, and BS. Then it can zoom part way in to measure large cells like adiopocytes or osteoid seams. Then it an zoom all the way in to measure smaller cells like osteoblasts or osteocytes. All this combines to make data collection fast.

Camera Presets

Camera presets make is simple to switch between brightfield, polarized light, and fluorescence imaging.

Presets store exposure time, gain, and white balance settings to standardize imaging and reduce setup times.


Reason #5: Faster Protocol Setup

Settings Assigned Arrays
Most settings in BIOQUANT have an Assign button next to them. Clicking that button stores the current value of that setting into the array. Then, at any time in the future, clicking the array returns the setting to its assigned value.

Settings Assigned Datasets
Files used with the whole data set like Guides, Selected Lists, Calculation Lists, and Measurement Filters can be assigned to a dataset. Default assignments have already been made to every template dataset in BIOQUANT.


Reason #6: Faster Training

Built-in Video Tutorial

Each region of the tool ribbon has a play button for video tutorials explaining how to use the tools. Additional videos are embedded in procedures in the PDF manual.

Guide Region rEDESIGN

The modern look of BIOQUANT OSTEO has a "ribbon" across the top of the screen. Regions of the ribbon are used left to right to complete a protocol.

The first region of the ribbon is the "Guide" region. A guides is a standard protocol for collecting a specific kind of data with BIOQUANT.

All BIOQUANT templates have guides to explain the protocol, You can also create custom guides faster then ever with the new Guide Editor.


PDF Manual with Tutorial Video

The manual for BIOQUANT OSTEO combines written protocols and embedded video. The manual is available as a PDF for the desktop.

This makes it simple for new lab members to get started with BIOQUANT. Video, consistent workflow, and guides combine to make it easy to get productive with BIOQUANT.


Reason #7: Faster Referent Data

Simultaneous and Instant Measurement
In one step, BIOQUANT automatically measures all referent data: TV, BV, BS, Tb.N, Tb.Sp, and Tb.Dm. This remarkable ability is possible because the software automates many other tasks.

Automatically Fill Cracks in the Bone
BIOQUANT automatically fills in small voids created by sectioning and staining artifacts. Large voids with cellular surfaces are preserved.

Automatically Exclude Extra Surfaces
BIOQUANT automatically excludes extra bone surface created when a trabeculum crosses the edge of the tissue volume.

Automatically Create the ROI for Cellular Measurements
The tissue volume automatically becomes the region of interest when you zoom into cellular magnification to measure osteoblast and osteoclast data


Reason #8: Faster Cellular Data


Simultaneous and Instantaneous Measurement
The Osteoid tool now automatically and simultaneously measures OV, Ob.S, O.Wi of multiple osteoid seams.

Automatically Identify the Surface
The Osteoid tool automatically finds the ends of the surface. It's no longer necessary to mark the ends manually.

Assign Arrays to the Osteoid Tool
The Width, Surface, and Volume arrays used in the Osteoid tool are assigned to the Selected array making it easy to measure different types of osteoid surfaces in one data set.


Simultaneous and Instantaneous Measurement
The Auto Surface tool automatically and simultaneously measures osteoclast data Oc.S and Oc.N.

Automatic Surface Computation
The Auto Surface tool looks at a thresholded object and computes the surface that the object (usually an osteoclast) occupies. This is not just 1/2 the perimeter, but the actual length of the object.


Automatic Single Label Surface
Using the Auto Surface tool, single label surface is automatically measured. The tool computes the length along the middle of the label. Threshold editing tools can quickly separate the single label from adjacent double label.

Simultaneous Double Label Surface and Interlabel Width Data
Hand drawing of the double label pairs is quick and BIOQUANT automatically measures the length of the double label and the interlabel distance along the surface.

Assign Arrays to the dLS Tool
The Width and Surface arrays are now assigned to the Selected array, allowing customization of the dLS Type for different array types.


New Measure Cells Tool
This tool automatically counts cells within a defined area, automatically filters out partial cells cut by the image or measurement area, and prevents double counting in overlapping fields of view. Measure Cells supports Auto ROI, so only cells within the Sampling region are measured at high magnification.

Automatic ROI
The ROI now changes shape automatically based on the current field of view and the sampling area tracing. You never have to use Topo ROI again.

Pan Box Snap to ROI
The Pan Box of the Large Image Navigator now snaps to the ROI. This means you can just click the Coarse Move Pan Box to move sequentially through the ROI and count cells without any fear of counting outside the ROI.


Reason #9: Faster Help with Technical Service Plans


Labs with active Technical Service Plans receive on-demand training via remote desktop sharing. Use this service to train new students, learn new software features, and design new data collection protocols.

Priority technical support

Get quick solutions to software and hardware problems by phone, email, or remote desktop.

Continuous annual upgrades

Each year, BIOQUANT publishes an upgrade to BIOQUANT OSTEO that is included at no charge as part of the Technical Services Plan. New versions add features requested by the community, consolidate protocol steps, and introduce new types of measurement tools.


Reason #10: Everything. Is. Faster.

90 Seconds per Trichrome Section for Referent Data
TV, BV, BS, BV/TV, BS/BV, Tb.N, Tb.Sp. Tb.Dm

10 Seconds per Trichrome Osteoid Seam for Osteoblast Data
OV, O.Wi, Ob.S, Ob.N

120 Seconds per TRAP Section for Osteoclast Data
Oc.S, Oc.N

5 Seconds per Calcein Double Label Surface for Formation Rate Data